CD8αα expression on NK cells is associated with different K562 and MOLT4 killing capabilities of PBMC and different involvement CD8pos and CD8neg subsets in anti-viral response

Introduction. The CD8αα are present in a subset of T cells and NK cells, but its function is mostly unknown, as well the role CD8+ CD8- cell subsets physiological pathological environments. Methods. We investigated 130 healthy individuals’ blood samples for cytotoxicity against K562 MOLT4 lines. also analyzed patients after SarsCov2 infection compared to control. phenotype were studied by FACScan flow cytometer using BD monoclonal antibodies. Results. confirmed that significantly more resistant “classical” K562. effective at killing subsets. correlation lymphocyte levels with specific lysis was weaker (r = 0.37) than 0.45) or whole population 0.46). However, we found did not participate MOLT-4 killing. frequency correlates 0.49) lymphocytes 0.27) 0.44). Also, showed HLA-DR CD158a positive correlate killing, while negative same significance population. Decreased results decrease owing CD8+NK decreased CD8neg. Conclusion. numbers determine indirectly through surface phenotype. CD8 expression on associated time obstructs response MOLT4. might HLA recognition enhance class-I target cells.